Expression of MAGE 3 Gene Products in Uterine Cervical Carcinoma / 대한산부인과학회잡지

OBJECTIVE: The human MAGE 3 gene encodes tumor specific antigens that are recognized by autologue cytotoxic T lymphocytes (CTL). The MAGE 3 gene is expressed not only in melanoma but in the other malignant tumors as well. There is, however, little information on the expression of the gene in uterine cervical carcinomas. The author thus studied the expression of the MAGE 3 gene products in uterine cervical carcinoma and discuss the possibility of specific immunologic diagnosis using MAGE 3 gene products. METHODS: The expression of MAGE 3 gene product in 17 normal tissues of the cervix, 32 cervical intraepithelial neoplasia (8 CIN I, 10 CIN II, 14 CIS), and 43 invasive cervical carcinomas was studied by immunohistochemistry using anti-MAGE 3 mAb 57B in paraffin sections RESULTS: No expression of MAGE 3 gene product was detected in normal cervical tissues and in cervical intraepithelial neoplasias. The expression of MAGE 3 gene product was detected in 30.2% (13/43) of invasive cervical carcinomas. The MAGE 3 gene product was stained as a cytoplasmic protein in cancer cells. No statistically significant differences were observed between MAGE 3 gene product expression status and clinicopathologic parameters. CONCLUSIONS: The MAGE 3 gene products was expressed in invasive cervical carcinoma tissues.

Dectection of Ureaplasma urealyticum in Invasive Cervical Cancer Tissue / 대한산부인과학회잡지

OBJECTIVE: Mycoplasmas have been implicated in many diseases including cervicitis, urethritis, salpingitis, endometritis... and functioning as cofactors catalyzing the HIV disease state. The oncogenic potentiality of mycoplasma was only recently realized when they were shown causing chromosomal changes and in vitro cell transformations through gradual progressive chromsomal loss and translocation. Few study has been reported the prevalence of mycoplasma infection in human cancers and suggested that there was a connection between these organisms and human cancers. The objective of this study was to determine the relationship between Ureaplasma urealyticum infection and cervical cancer. METHODS: The detection frequency of Ureaplasma urealyticum in 52 invasive cervical cancer tissues and 17 normal cervical tissues was studied using PCR. RESULTS: U. urealyticum DNA was detected in 8 out of 52(15.4%) invasive cervical cancer tissues and 1 out of 17(5.9%) normal cervical tissues. No statistic significance was observed between the detection frequency of Ureaplasma urealyticum and clinicopathologic parameters. The prevalence of Ureaplasma urealyticum in invasive cervical tissues was 15.4% and this rate was higher than 5.9% in normal cervical tissues but there was no statistic significance. CONCLUSIONS: With respect to clinicopathologic parameters of cervical cancer, there was no significant relation between U. urealyticum infection and cervical cancer. There is, however, few study and case on cervical cancer internally and externally. It is considered that more studies on the subject with much cases should be made.

Detection and culture of Ureaplasma urealyticum in Ovarian Cancer / 대한산부인과학회잡지

BACKGROUND & OBJECTIVES:According to recent studies, Mycoplasma DNA was positive in 20% of uterine cervical cancer tissue and 48% of gastric cancer tissue. It also showed the correlation with human cancers and infection of Mycoplasma. Ureaplasma urealyticum(U. urealyticum) is a bacteria of high infection rate in women's urogenital system, but it is difficult to find a report about the relations to cancer. So, the objectives of this studies are to investigate the infection rate of the U. urealyticum from Korean women's ovarian cancer, borderline ovarian cancer and benign ovarian tumor, and to discover the relationship between these ovarian tumors with U. urealyticum's infection. METHODS: In obstetrics and gynecology department of Kosin medical college, the PCR procedure and cultivation was performed on extracted cancer tissues of 22 ovarian cancer patients and 5 borderline ovarian cancer patients who had operation, and it was also performed on 30 benign ovarian tumor patients as an control group. RESULTS: The detection freguency of U. urealyticum in ovarian cancer tissue was 31.8% (7/22) which was higher than 13.3% (4/30) of benign ovarian tumor but had no statistical relation (p=0.318). In borderline ovarian cancer, the detection frequency was 0.0% (0/5). The infection frequency of U. urealyticum was 22.7% (5/22) in ovarian cancer tissue and 0.0% (0/5) in borderline ovarian tumor and 3.3% (1/30) in benign ovarian tumor, so the infection frequency was higher in ovarian cancer tissue than benign ovarian tumor which was showing statistical relation (p=0.024). The detection frequency of U. urealyticum from benign ovarian tumor tissue was 23.0% (3/13) in mature teratoma and 16.7% (1/6) in epithelial ovarian tumor, which was showing higher frequency in mature teratoma but had no statistical relation(p=0.589). The infection frequency was showed only in epithelial ovarian tumor as 16.7% (1/6) but also had no statistical relation(p=0.388). The detection frequency of U. urealyticum followed by the FIGO stage of malignant ovarian cancer was 33% in stage I and 30.8% (4/13) in stage III, which was higher in stage I but had no statistical relation(p=0.662). The infection frequency was 11.1% (1/9) in stage I and 30.8% (4/13) in stage III, which was higher in stage III but had no statistical relation(p=0.360). CONCLUSION: With the above results, there was no significant relation between U. urealyticum infection and ovarian cancer. There is, however, few study and case on ovarian cancer internally and externally. It is considered that more studies on the subject with much cases should be made.

A Clinicopathologic Study of 31 Cases with Ovarian Malignant Germ Cell Tumors / 대한산부인과학회잡지

OBJECTIVE: The purpose of this study was to review the clinicopathologic features, recurrent rate, survival rate and controversable issues in the treatment of the ovarian malignant germ cell tumors. PATIENTS AND METHODS: From August, 1991 to November, 1998 thirty-one patients with malignant germ cell tumors of the ovary treated in the department of obstetrics and gynecology, Kosin University Medical college, were eligible and assessable. Demographic characteristics, symptoms, signs, stage, tumor grade, mode of therapy and results of follow up were reviewed retrospectively. RESULTS: The patients with malignant germ cell tumor constituted 6.37% of all ovarian malignancies during this period. Histologic subtypes were 8 dysgerminoma(25.8%), 7 endodermal sinus tumor(22.6%), 10 immature teratoma(32.3%), 3 mixed germ cell tumor(9.7%), 3 choriocarcinoma(9.7%). The age of the patients ranged from 10 to 40 years (mean +/-S.D.; 24.26 +/- 7.51). The most common symptom was abdominal pain(38.7%). Most had stageI(18 cases, 58.0%) or stageIII(5 cases, 16.2%) diseases. All patients underwent surgery as the initial treatment, and nine patients received more than one operation. Postoperative adjuvant chemotherapeutic regimens were VAC, VBP, EP, BEP, EMA, and EMA CO. The mean follow up duration was 26.0(+/- S.D.; +/- 20.3) months. The 2-year and 5-year survival rate were 91.97%(+/- S.E.; +/- 0.05) and 86.86%(+/- S.E.; +/- 0.07).

Expression of bcl-2 and p53 Protein in Invasive Cervical Cancer / 대한부인종양콜포스코피학회잡지

Recently, the bcl-2 and p53 protein have been recognized as important factors that is contributed to programmed cell death. The objective of this study was to evaluate the prognostic significance of bcl-2 and p53 protein expression in uterine cervical carcinoma. The expression of bcl-2 and p53 in 59 cases of uterine cervical carcinoma (stage IB to IIB) were surgically treated from January 1993 to June 1994. The expression of bcl-2 and p53 was examined by immunohistochemical method using formalin fixed paraffin embedded tissue specimens. The 48 cases were squamous cell carcinoma and 11 cases were adenocarcinoma. The results were as follows: 1. The expression rate of bcl-2 protein was 28.8%(17/59) and there was no significant correlaltion between the expression of bcl-2 protein and the clinicopathologic parameters (histologic type, grade, FIGO stage, cervical invasion depth, lymph node metastasis, parametrial invasion, tumor size, neoadjuvant chemotherapy response, recurrence, survival). 2. The expression rate of p53 protein was 32.2%(19/59) and there was no significant correlation between expression of p53 protein and the clinicopathologic parameters. 3. There was significant correlation between and expression of bcl-2 and p53 protein (P 0.05). In conclusion, bcl-2 and p53 protein are thought to be possible factors in the carcinogenesis of uterine cervical carcinoma and correlate with progression of it. But further study will be required to clarify the role of bcl-2 and p53 in carcinogenesis of the uterine cervix.